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Expression of barley HvCBF4 enhances tolerance to abiotic stress in transgenic rice.
- Source :
-
Plant Biotechnology Journal . Sep2007, Vol. 5 Issue 5, p646-656. 11p. 1 Color Photograph, 3 Black and White Photographs, 1 Diagram, 2 Charts, 1 Graph. - Publication Year :
- 2007
-
Abstract
- C-repeat/dehydration-responsive element binding factors (CBF/DREBs) are a family of transcription factors that regulate freezing tolerance in Arabidopsis. As a step towards understanding the stress response of monocotyledonous plants, we isolated a barley gene HvCBF4 whose expression is induced by low-temperature stress. Transgenic over-expression of HvCBF4 in rice resulted in an increase in tolerance to drought, high-salinity and low-temperature stresses without stunting growth. Interestingly, under low-temperature conditions, the maximum photochemical efficiency of photosystem II in the dark-adapted state ( Fv/ Fm, where Fv is the variable fluorescence and Fm is the maximum fluorescence) in HvCBF4 plants was higher by 20% and 10% than that in non-transgenic and CBF3/DREB1A plants, respectively. Using the 60K Rice Whole Genome microarray, 15 rice genes were identified that were activated by HvCBF4. When compared with 12 target rice genes of CBF3/DREB1A, five genes were common to both HvCBF4 and CBF3/DREB1A, and 10 and seven genes were specific to HvCBF4 and CBF3/DREB1A, respectively. Interestingly, HvCBF4 did not activate Dip1 and Lip5, two important target genes of CBF3/DREB1A, in transgenic rice under normal growth conditions, but their expression was enhanced by HvCBF4 under low-temperature conditions. Our results suggest that CBF/DREBs of barley act differently from those of Arabidopsis in transgenic rice. [ABSTRACT FROM AUTHOR]
Details
- Language :
- English
- ISSN :
- 14677644
- Volume :
- 5
- Issue :
- 5
- Database :
- Academic Search Index
- Journal :
- Plant Biotechnology Journal
- Publication Type :
- Academic Journal
- Accession number :
- 25958995
- Full Text :
- https://doi.org/10.1111/j.1467-7652.2007.00272.x