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Antigen-independent and antigen-dependent methods to numerically expand CD19-specific CD8+ T cells
- Source :
-
Experimental Hematology . Jul2007, Vol. 35 Issue 7, p1083-1090. 8p. - Publication Year :
- 2007
-
Abstract
- Objective: Preclinical and clinical trials are investigating the potential of T cells genetically modified to express a first-generation CD19-specific chimeric antigen receptor (CAR), designated CD19R, for adoptive immunotherapy of B-lineage leukemias and lymphomas. Currently, our genetically modified CD19-specific CD8+ (CD19R+CD8+) T cells are expanded ex vivo using a rapid expansion protocol (REP) to clinically meaningful numbers after antigen-independent activation with anti-CD3ɛ and recombinant human interleukin-2 on a double-cell feeder-layer of γ-irradiated allogeneic peripheral blood mononuclear cells and a lymphoblastoid cell line. We now compare the ability of the REP with CD19-dependent numerical expansion using CD19+ artificial antigen-presenting cells to propagate CD19R+CD8+ T cells. Materials and Methods: We evaluated long-term (28 days) propagation, CD19R CAR expression, and cytolytic activity of CD19R+CD8+ T cells expanded by either a REP or an antigen expansion protocol (AEP) using K562-derived artificial antigen-presenting cells coexpressing CD19 antigen and two T-cell costimulatory molecules (4-1BB ligand and major histocompatibility class I–related chains A) in the presence of exogenous recombinant human interleukin-2 and recombinant human interleukin-15. Results: Populations of CD19R+CD8+ T cells could be numerically expanded on AEP to meet anticipated clinical need. The AEP was superior to REP, as this method selected for an outgrowth of T cells with increased CD19R CAR expression and improved redirected cytolytic activity. Conclusion: Robust propagation of CD19R+CD8+ T cells achieved by AEP supports qualifying this cell line for use in current good manufacturing practices for CAR+ T cells as an alternative to REP for adoptive immunotherapy clinical trials. [Copyright &y& Elsevier]
- Subjects :
- *T cells
*LYMPHOCYTES
*MEDICAL experimentation on humans
*MEDICAL research
Subjects
Details
- Language :
- English
- ISSN :
- 0301472X
- Volume :
- 35
- Issue :
- 7
- Database :
- Academic Search Index
- Journal :
- Experimental Hematology
- Publication Type :
- Academic Journal
- Accession number :
- 25501335
- Full Text :
- https://doi.org/10.1016/j.exphem.2007.04.007