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Role of glutamate decarboxylase (GAD) isoform, GAD65, in GABA synthesis and transport into synaptic vesicles—Evidence from GAD65-knockout mice studies
- Source :
-
Brain Research . Jul2007, Vol. 1154, p80-83. 4p. - Publication Year :
- 2007
-
Abstract
- Abstract: In GAD65-knockout mice, lack of GAD65 expression was confirmed. The expression level of vesicular GABA transporter (VGAT) was upregulated, and no change in the synaptic vesicles (SV)-associated GAD67 was found. GAD65(−/−) SV transported cytosolic GABA much more efficiently than that of the wild type, further supporting our model that there is a structural and functional coupling between GABA synthesis and packaging into SV. Both full-length and truncated forms of GAD65 could bind to GABAergic SV, indicating the N-terminus is not required for the anchoring of GAD65 to SV. Although both GAD65(−/−) SV reconstituted with either GAD65 or GAD67 could synthesize GABA from [3H] glutamate and transport this newly synthesized GABA into SV, the combined evidence suggests that GAD65 plays a major role in GABA transmission in normal physiological condition. However, GAD67 could serve this role under some pathological conditions. [Copyright &y& Elsevier]
- Subjects :
- *GLUTAMATE decarboxylase
*GABA
*COATED vesicles
*AMINO acid neurotransmitters
Subjects
Details
- Language :
- English
- ISSN :
- 00068993
- Volume :
- 1154
- Database :
- Academic Search Index
- Journal :
- Brain Research
- Publication Type :
- Academic Journal
- Accession number :
- 25330268
- Full Text :
- https://doi.org/10.1016/j.brainres.2007.04.008