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Suppression of matrix metalloproteinase-9 by 4-methylumbelliferone
- Source :
-
Cell Biology International . Sep2007, Vol. 31 Issue 9, p1022-1026. 5p. - Publication Year :
- 2007
-
Abstract
- Abstract: OHK cells, a human lymphoma cell line, are known to produce large amounts of hyaluronan. We investigated the effect of 4-methylumbelliferone, an inhibitor of hyaluronan synthesis, on the activity of matrix metalloproteinases in OHK cells. Matrix metalloproteinase-9 was detected on gelatin zymography as the main metalloproteinase excreted into the medium of cultured OHK cells, and 4-methylumbelliferone added to the medium decreased the activity of the enzyme in a dose-dependent manner. Addition of Streptomyces hyaluronidase to the medium during cultivation did not decrease the enzyme activity. Reverse transcription-polymerase chain reaction revealed that 4-methylumbelliferone markedly decreased the level of mRNA for matrix metalloproteinase-9 in cultured OHK cells. A similar decrease of the activity of matrix metalloproteinase-9 by 4-methylumbelliferone was also observed in cultured human breast and colon carcinoma cells. These results suggest that 4-methylumbelliferone suppresses the expression of matrix metalloproteinase-9 in cultured cancer cells. [Copyright &y& Elsevier]
- Subjects :
- *CELL lines
*HYALURONIC acid
*METALLOENZYMES
*DNA polymerases
Subjects
Details
- Language :
- English
- ISSN :
- 10656995
- Volume :
- 31
- Issue :
- 9
- Database :
- Academic Search Index
- Journal :
- Cell Biology International
- Publication Type :
- Academic Journal
- Accession number :
- 25255645
- Full Text :
- https://doi.org/10.1016/j.cellbi.2007.03.016