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Cytokine regulation of MMP-9 in peripheral glia: Implications for pathological processes and pain in injured nerve
- Source :
-
Brain, Behavior & Immunity . Jul2007, Vol. 21 Issue 5, p561-568. 8p. - Publication Year :
- 2007
-
Abstract
- Abstract: Matrix metalloproteinase-9 (MMP-9) is an extracellular protease that is induced in Schwann cells hours after peripheral nerve injury and controls axonal degeneration and macrophage recruitment to the lesion. Here, we report a robust (90-fold) increase in MMP-9 mRNA within 24h after rat sciatic nerve crush (1 to 60 days time-course). Using direct injection into a normal sciatic nerve, we identify the proinflammatory cytokines TNF-α and IL-1β as potent regulators of MMP-9 expression (Taqman qPCR, zymography). Myelinating Schwann cells produced MMP-9 in response to cytokine injection and crush nerve injury. MMP-9 gene deletion reduced unstimulated neuropathic nociceptive behavior after one week post-crush and preserved myelin thickness by protecting myelin basic protein (MBP) from degradation, tested by Western blot and immunofluorescence. These data suggest that MMP-9 expression in peripheral nerve is controlled by key proinflammatory cytokine pathways, and that its removal protects nerve fibers from demyelination and reduces neuropathic pain after injury. [Copyright &y& Elsevier]
- Subjects :
- *CELLULAR immunity
*ALTERNATIVE medicine
*NERVOUS system
*MESSENGER RNA
Subjects
Details
- Language :
- English
- ISSN :
- 08891591
- Volume :
- 21
- Issue :
- 5
- Database :
- Academic Search Index
- Journal :
- Brain, Behavior & Immunity
- Publication Type :
- Academic Journal
- Accession number :
- 25255260
- Full Text :
- https://doi.org/10.1016/j.bbi.2006.10.015