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Cell labeling with the positive MR contrast agent Gadofluorine M.
- Source :
-
European Radiology . May2007, Vol. 17 Issue 5, p1226-1234. 9p. 3 Diagrams, 3 Graphs. - Publication Year :
- 2007
-
Abstract
- The purpose of this study was to label human monocytes with Gadofluorine M by simple incubation for subsequent cell depiction at 1.5 and 3 T. Gadofluorine M displays a high r(1) relaxivity and is spontaneously phagocytosed by macrophages. Human monocytes were incubated with Gadofluorine M-Cy at varying concentrations and incubation times and underwent MR imaging at 1.5 and 3 T at increasing time intervals after the labeling procedure. R1-relaxation rates and r1 relaxivities of the labeled cells and non-labeled controls were determined. Cellular contrast agent uptake was examined by fluorescence microscopy and quantified by ICP-AES. Efficient cell labeling was achieved after incubation of the cells with 25 mM Gd Gadofluorine M for 12 h, resulting in a maximal uptake of 0.3 fmol Gd/cell without impairment of cell viability. Fluorescence microscopy confirmed internalization of the fluorescent contrast agent by monocytes. The r1 relaxivity of the labeled cells was 137 mM(-1)s(-1) at 1.5 T and 80.46 mM(-1)s(-1) at 3 T. Imaging studies showed stable labeling for at least 7 days. Human monocytes can be effectively labeled for MR imaging with Gadofluorine M. Potential in vivo cell-tracking applications include targeting of inflammatory processes with Gadofluorine-labeled leukocytes or monitoring of stem cell therapies for the treatment of arthritis. [ABSTRACT FROM AUTHOR]
- Subjects :
- *MONOCYTES
*FLUORESCENCE microscopy
*MACROPHAGES
*PSYCHOANALYSIS
*RADIOSCOPIC diagnosis
*MEDICAL radiography
*RADIOGRAPHY
*CELL culture
*COMPARATIVE studies
*MAGNETIC resonance imaging
*RESEARCH methodology
*MEDICAL cooperation
*MICROSCOPY
*ORGANOMETALLIC compounds
*RESEARCH
*RESEARCH funding
*SPECTROPHOTOMETRY
*STAINS & staining (Microscopy)
*EVALUATION research
*CONTRAST media
Subjects
Details
- Language :
- English
- ISSN :
- 09387994
- Volume :
- 17
- Issue :
- 5
- Database :
- Academic Search Index
- Journal :
- European Radiology
- Publication Type :
- Academic Journal
- Accession number :
- 24939735
- Full Text :
- https://doi.org/10.1007/s00330-006-0522-9