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Determination of Huperzine A in rat plasma by high-performance liquid chromatography with a fluorescence detector
- Source :
-
Journal of Pharmaceutical & Biomedical Analysis . May2007, Vol. 44 Issue 1, p309-312. 4p. - Publication Year :
- 2007
-
Abstract
- Abstract: A simple reversed-phase high-performance liquid chromatography (HPLC)-fluorescence method for the determination of Huperzine A in rat plasma was developed and validated. Separation was achieved on Kromasil C(8) column (5μm, 150mm×4.6mm i.d.). The mobile phase, methanol–water–triethanol amine (45:55:0.05, v/v/v), was delivered at a flow rate of 1.0ml/min. The eluent was monitored by a fluorescence detector with excitation wavelength at 310nm and emission wavelength at 370nm. No interfering peaks were observed in rat blank plasma. The relationship between Huperzine A concentration and peak-area ratio of Huperzine A to the IS was linear over the range of 2.5–250ng/ml. The intra- and inter-day coefficients of variation were ≤10.1% and ≤14.1%, respectively. The method and extraction recovery of Huperzine A were 101.9–108.9% and 73.5–84.6%, respectively. Huperzine A was found to be stable for at least 5h at RT and 1 week at −20°C. The method was applied to a pharmacokinetic study of Huperzine A in rats following intranasal administration. [Copyright &y& Elsevier]
- Subjects :
- *LIQUID chromatography
*FLUORESCENCE
*AMINES
*WAVELENGTHS
Subjects
Details
- Language :
- English
- ISSN :
- 07317085
- Volume :
- 44
- Issue :
- 1
- Database :
- Academic Search Index
- Journal :
- Journal of Pharmaceutical & Biomedical Analysis
- Publication Type :
- Academic Journal
- Accession number :
- 24709926
- Full Text :
- https://doi.org/10.1016/j.jpba.2007.02.019