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Sequential Injection Determination of Nitrate in Vegetables by Spectrophotometry with Inline Cadmium Reduction.
- Source :
-
Communications in Soil Science & Plant Analysis . 2007, Vol. 38 Issue 3/4, p533-544. 12p. 1 Diagram, 3 Charts, 1 Graph. - Publication Year :
- 2007
-
Abstract
- A sequential injection system for the determination of nitrate (NO3-) in vegetables was developed to automate this determination, allowing for substantially reduced reagent consumption and generated waste using low-cost equipment. After extraction with water and filtration, the extracted nitrate is reduced inline to nitrite in a copperized cadmium (Cd) column and determined as nitrite. According to the Griess–Ilosvay reaction, nitrate is diazotized with sulfanilamide and coupled with N-(1-naphtyl)-ethylenediamine dihydrochloride to form a purple-red azo dye monitored at 538 nm. Nitrate can be determined within a range of 1.35–50.0 mg L-1 of NO3- (corresponding to 0.270–10.0 g of NO3- per kg of vegetable), with a conversion rate of nitrate to nitrite of 99.1±0.8%. The results obtained for 15 vegetable extracts compare well with those provided by the classical procedure, with a sampling throughput of 24 determinations per hour and relative standard deviations better than 1.2%. [ABSTRACT FROM AUTHOR]
Details
- Language :
- English
- ISSN :
- 00103624
- Volume :
- 38
- Issue :
- 3/4
- Database :
- Academic Search Index
- Journal :
- Communications in Soil Science & Plant Analysis
- Publication Type :
- Academic Journal
- Accession number :
- 24156223
- Full Text :
- https://doi.org/10.1080/00103620601174635