Back to Search
Start Over
Anionic phospholipids inhibit apolipoprotein E—Low-density lipoprotein receptor interactions
- Source :
-
Biochemical & Biophysical Research Communications . Mar2007, p820-824. 5p. - Publication Year :
- 2007
-
Abstract
- Abstract: Apolipoprotein E (apoE) is a ligand for members of the low-density lipoprotein receptor (LDLR) family. Lipid-free apoE is not recognized by LDLR, yet interaction with lipid confers receptor recognition properties. Although lipid interaction is known to induce a conformational change in apoE, it is not known if the lipid composition of the resulting complex influences binding. Using reconstituted lipoprotein particles of apoE3 N-terminal (NT) domain and dimyristoylphosphatidylcholine (DMPC), maximal LDLR binding was observed at DMPC:apoE3-NT ratios >2.5:1 (w/w). ApoE3-NT lipid particles prepared with egg sphingomyelin were functional as LDLR ligands while complexes formed with the anionic phospholipids dimyristoylphosphatidylglycerol or dimyristoylphosphatidylserine (DMPS) were not. In the case of apoE3-NT, lipid particles comprised of a mixture of DMPC and DMPS, a DMPS concentration dependent inhibition of LDLR binding activity was observed. Thus, in addition to affecting apoE conformational status, the lipid composition of ligand particles can modulate LDLR binding activity. [Copyright &y& Elsevier]
- Subjects :
- *APOLIPOPROTEIN E
*PHOSPHOLIPIDS
*CELL receptors
*PROTEIN binding
Subjects
Details
- Language :
- English
- ISSN :
- 0006291X
- Database :
- Academic Search Index
- Journal :
- Biochemical & Biophysical Research Communications
- Publication Type :
- Academic Journal
- Accession number :
- 23876035
- Full Text :
- https://doi.org/10.1016/j.bbrc.2007.01.066