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Curcumin induces pro-apoptotic endoplasmic reticulum stress in human leukemia HL-60 cells
- Source :
-
Biochemical & Biophysical Research Communications . Feb2007, Vol. 353 Issue 4, p1040-1045. 6p. - Publication Year :
- 2007
-
Abstract
- Abstract: Curcumin has been shown to induce apoptosis in many cancer cells. However, the molecular mechanism(s) responsible for curcumin-induced apoptosis is not well understood and most probably involves several pathways. In HL-60 cells, curcumin induced apoptosis and endoplasmic reticulum (ER) stress as evidenced by the survival molecules such as phosphorylated protein kinase-like ER-resident kinase, phosphorylated eukaryotic initiation factor-2α, glucose-regulated protein-78, and the apoptotic molecules such as caspase-4 and CAAT/enhancer binding protein homologous protein (CHOP). Inhibition of caspase-4 activity by z-LEVD-FMK, blockage of CHOP expression by small interfering RNA, and treatment with salubrinal, an ER inhibitor, significantly reduced curcumin-induced apoptosis. Removing two double bonds in curcumin, which was speculated to form Michael adducts with thiols in secretory proteins, resulted in a loss of the ability of curcumin to induce apoptosis as well as ER stress. Thus, the present study shows that curcumin-induced apoptosis is associated with its ability to cause ER stress. [Copyright &y& Elsevier]
- Subjects :
- *APOPTOSIS
*CELL death
*CANCER cells
*ENDOPLASMIC reticulum
*PROTEINS
Subjects
Details
- Language :
- English
- ISSN :
- 0006291X
- Volume :
- 353
- Issue :
- 4
- Database :
- Academic Search Index
- Journal :
- Biochemical & Biophysical Research Communications
- Publication Type :
- Academic Journal
- Accession number :
- 23665923
- Full Text :
- https://doi.org/10.1016/j.bbrc.2006.12.133