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Sensitive and specific method for detecting G protein–coupled receptor mRNAs.
- Source :
-
Nature Methods . Jan2007, Vol. 4 Issue 1, p35-37. 3p. 2 Diagrams. - Publication Year :
- 2007
-
Abstract
- G protein–coupled receptors (GPCRs) mediate effects of extracellular signaling molecules in all the body's cells. These receptors are encoded by scarce mRNAs; therefore, detecting their transcripts with conventional microarrays is difficult. We present a method based on multiplex PCR and array detection of amplicons to assay GPCR gene expression with as little as 1 μg of total RNA, and using it, we profiled three human bone marrow stromal cell (BMSC) lines. [ABSTRACT FROM AUTHOR]
Details
- Language :
- English
- ISSN :
- 15487091
- Volume :
- 4
- Issue :
- 1
- Database :
- Academic Search Index
- Journal :
- Nature Methods
- Publication Type :
- Academic Journal
- Accession number :
- 23547145
- Full Text :
- https://doi.org/10.1038/nmeth977