A sequential injection analysis/chemiluminescent plant tissue-based biosensor system for the determination of diamine

Abstract: In this paper, a new chemiluminescent plant tissue-based biosensor for diamine detection was presented by employing sequential injection analysis (SIA), which facilitates precise fluidic handling and lower consumption of sample and reagents. Pea-seedling tissue acted as the molecular recognition element and was packed in a mini-PTFE column and further incorporated in the SIA system. The analysis of diamines, such as putrescine and cadaverine, is based on an enzymatic conversion which takes place in the plant tissue column to produce hydrogen peroxide. The formed hydrogen peroxide was detected by a chemiluminescence reaction involving luminol and Co2+. Under the optimal conditions, the linear calibration graphs were obtained within 0.2–80μM (putrescine) and 0.5–100μM (cadaverine). The detection limits of 0.03 and 0.06μM were achieved for putrescine and cadaverine, respectively, along with the relative standard deviations of 2.14% and 3.08% (n =11) and a sampling frequency of 40h−1. The present biosensor has been used for the analysis of diamine in fish samples with an acceptable accuracy. [Copyright &y& Elsevier]