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Analysis of the cytokine profile in spleen cells from dogs naturally infected by Leishmania chagasi
- Source :
-
Veterinary Immunology & Immunopathology . Jan2007, Vol. 115 Issue 1/2, p135-145. 11p. - Publication Year :
- 2007
-
Abstract
- Abstract: Recent studies suggest that asymptomatic dogs infected with canine visceral leishmaniasis (CVL) develop a Th1 immunological profile whilst oligosymptomatic and symptomatic CVL-infected animals present a Th2 profile. In the present study, an RT-PCR method has been standardised and employed to evaluate the frequency and the semi-quantitative level of expression of the cytokines IL-4, IL-10, IL-12, INF-γ and TNF-α in splenocytes of 30 dogs naturally infected with Leishmania chagasi and of 7 non-infected dogs (NID). An increase in the level of expression of IL-12 (p =0.059) was detected in all CVL-infected dogs compared with NID. In dogs exhibiting high parasitism, the frequency of expression of IL-10 was higher (p =0.011) than in animals presenting low parasitism or medium parasitism (MP) and in NID animals, whilst the level of expression of IL-10 was higher (p =0.0094) than in animals exhibiting MP and in the NID group. Positive correlations between the levels of expression of IL-10 with respect to the progression of the disease (IL-10: r =0.3510; p =0.0337) and the levels of expression of IL-10 and INF-γ increase in parasitism (IL-10: r =0.3428; p =0.0438 and INF-γ: r =0.4690; p =0.0045) were observed. Such data suggest that CVL is marked by a balanced production of Th1 and Th2 cytokines, with a predominant accumulation of IL-10 as a consequence of an increase in parasitic load and progression of the disease, and INF-γ was related with the increase in parasitic load. [Copyright &y& Elsevier]
- Subjects :
- *CYTOKINES
*CELLULAR immunity
*LYMPHOID tissue
*PROTOZOAN diseases
Subjects
Details
- Language :
- English
- ISSN :
- 01652427
- Volume :
- 115
- Issue :
- 1/2
- Database :
- Academic Search Index
- Journal :
- Veterinary Immunology & Immunopathology
- Publication Type :
- Academic Journal
- Accession number :
- 23445539
- Full Text :
- https://doi.org/10.1016/j.vetimm.2006.10.001