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Requirement for Annexin Al in Plasma Membrane Repair.
- Source :
-
Journal of Biological Chemistry . 11/17/2006, Vol. 281 Issue 46, p35202-35207. 6p. 4 Graphs. - Publication Year :
- 2006
-
Abstract
- Ca2+ entering a cell through a torn or disrupted plasma membrane rapidly triggers a combination of homotypic and exocytotic membrane fusion events. These events serve to erect a reparative membrane patch and then anneal it to the defect site. Annexin Al is a cytosolic protein that, when activated by micromolar Ca2+, binds to membrane phospholipids, promoting membrane aggregation and fusion. We demonstrate here that an annexin A1 function-blocking antibody, a small peptide competitor, and a dominant-negative annexin A1 mutant protein incapable of Ca2+ binding all inhibit resealing. Moreover, we show that, coincident with a resealing event, annexin Al becomes concentrated at disruption sites. We propose that Ca2+ entering through a disruption locally induces annexin Al binding to membranes, initiating emergency fusion events whenever and wherever required. [ABSTRACT FROM AUTHOR]
Details
- Language :
- English
- ISSN :
- 00219258
- Volume :
- 281
- Issue :
- 46
- Database :
- Academic Search Index
- Journal :
- Journal of Biological Chemistry
- Publication Type :
- Academic Journal
- Accession number :
- 23420042
- Full Text :
- https://doi.org/10.1074/jbc.M606406200