Back to Search
Start Over
Pullulan production by tropical isolates of Aureobasidium pullulans.
- Source :
-
Journal of Industrial Microbiology & Biotechnology . Jan2007, Vol. 34 Issue 1, p55-61. 7p. 4 Charts, 2 Graphs. - Publication Year :
- 2007
-
Abstract
- Tropical isolates of Aureobasidium pullulans previously isolated from distinct habitats in Thailand were characterized for their capacities to produce the valuable polysaccharide, pullulan. A. pullulans strain NRM2, the so-called “color variant” strain, was the best producer, yielding 25.1 g pullulan l−1 after 7 days in sucrose medium with peptone as the nitrogen source. Pullulan from strain NRM2 was less pigmented than those from the other strains and was remarkably pure after a simple ethanol precipitation. The molecular weight of pullulan from all cultures dramatically decreased after 3 days growth, as analyzed by high performance size exclusion chromatography. Alpha-amylase with apparent activity against pullulan was expressed constitutively in sucrose-grown cultures and induced in starch-grown cultures. When the alpha-amylase inhibitor acarbose was added to the culture medium, pullulan of slightly higher molecular weight was obtained from late cultures, supporting the notion that alpha-amylase plays a role in the reduction of the molecular weight of pullulan during the production phase. [ABSTRACT FROM AUTHOR]
Details
- Language :
- English
- ISSN :
- 13675435
- Volume :
- 34
- Issue :
- 1
- Database :
- Academic Search Index
- Journal :
- Journal of Industrial Microbiology & Biotechnology
- Publication Type :
- Academic Journal
- Accession number :
- 23368457
- Full Text :
- https://doi.org/10.1007/s10295-006-0163-7