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Involvement of Ca2+ in the inhibition by crocetin of angiotensin II-induced ERK1/2 activation in vascular smooth muscle cells

Authors :
Zhou, Cheng-Hua
Qian, Zhi-Yu
Xiang, Min
He, Shu-Ying
Source :
European Journal of Pharmacology. Jan2007, Vol. 554 Issue 2/3, p85-91. 7p.
Publication Year :
2007

Abstract

Abstract: Crocetin, a carotenoid compound, was isolated from Gardenia jasminoids Ellis. Our recent study shows that crocetin inhibits angiotensin II-induced extracellular signal-regulated kinases 1/2 (ERK1/2) activation and subsequent proliferation in vascular smooth muscle cells (VSMCs). To further explore the mechanism involved, in the present study, we investigated the effect of Ca2+ in the activation of ERK1/2 and whether Ca2+ is involved in the suppression by crocetin of angiotensin II-induced ERK1/2 activation. Our findings showed that crocetin pretreatment partially attenuated both the intracellular Ca2+ mobilization and the extracellular Ca2+ influx induced by angiotensin II. Moreover, angiotensin II-induced ERK1/2 activation was completely abolished by acetoxymethyl ester of 1,2-bis(2-aminophenoxy)ethane-N,N,N ′,N′-tetraacetic acid (BAPTA-AM), an intracellular Ca2+ chelator, and partially inhibited by EGTA, an extracellular Ca2+ chelator, or verapamil, an L-type Ca2+ channel blocker. These findings suggest that Ca2+ may play an important role in angiotensin II-induced ERK1/2 activation in VSMCs, and Ca2+-dependent pathway may be involved in the inhibitory effect by crocetin of angiotensin II-induced ERK1/2 activation. [Copyright &y& Elsevier]

Details

Language :
English
ISSN :
00142999
Volume :
554
Issue :
2/3
Database :
Academic Search Index
Journal :
European Journal of Pharmacology
Publication Type :
Academic Journal
Accession number :
23352715
Full Text :
https://doi.org/10.1016/j.ejphar.2006.09.069