Back to Search
Start Over
Involvement of Ca2+ in the inhibition by crocetin of angiotensin II-induced ERK1/2 activation in vascular smooth muscle cells
- Source :
-
European Journal of Pharmacology . Jan2007, Vol. 554 Issue 2/3, p85-91. 7p. - Publication Year :
- 2007
-
Abstract
- Abstract: Crocetin, a carotenoid compound, was isolated from Gardenia jasminoids Ellis. Our recent study shows that crocetin inhibits angiotensin II-induced extracellular signal-regulated kinases 1/2 (ERK1/2) activation and subsequent proliferation in vascular smooth muscle cells (VSMCs). To further explore the mechanism involved, in the present study, we investigated the effect of Ca2+ in the activation of ERK1/2 and whether Ca2+ is involved in the suppression by crocetin of angiotensin II-induced ERK1/2 activation. Our findings showed that crocetin pretreatment partially attenuated both the intracellular Ca2+ mobilization and the extracellular Ca2+ influx induced by angiotensin II. Moreover, angiotensin II-induced ERK1/2 activation was completely abolished by acetoxymethyl ester of 1,2-bis(2-aminophenoxy)ethane-N,N,N ′,N′-tetraacetic acid (BAPTA-AM), an intracellular Ca2+ chelator, and partially inhibited by EGTA, an extracellular Ca2+ chelator, or verapamil, an L-type Ca2+ channel blocker. These findings suggest that Ca2+ may play an important role in angiotensin II-induced ERK1/2 activation in VSMCs, and Ca2+-dependent pathway may be involved in the inhibitory effect by crocetin of angiotensin II-induced ERK1/2 activation. [Copyright &y& Elsevier]
- Subjects :
- *ANGIOTENSIN II
*SMOOTH muscle
*MUSCLE cells
*CALCIUM
Subjects
Details
- Language :
- English
- ISSN :
- 00142999
- Volume :
- 554
- Issue :
- 2/3
- Database :
- Academic Search Index
- Journal :
- European Journal of Pharmacology
- Publication Type :
- Academic Journal
- Accession number :
- 23352715
- Full Text :
- https://doi.org/10.1016/j.ejphar.2006.09.069