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Effect of glutamine on glutathione kinetics in vivo in dogs
- Source :
-
Journal of Nutritional Biochemistry . Jan2007, Vol. 18 Issue 1, p10-16. 7p. - Publication Year :
- 2007
-
Abstract
- Abstract: To determine whether glutamine affects glutathione (GSH, γ-glutamyl-cysteinyl-glycine) metabolism, seven healthy beagle dogs received 6-h infusions of [15N]glutamate and [13C]leucine after a 3-day fast. Isotope infusions were performed during oral feeding with an elemental regimen, supplemented with either l-glutamine or an isonitrogenous amino acid mixture, on two separate days and in randomized order. Timed blood samples were obtained, and a surgical duodenal biopsy was performed after 6 h of isotope infusion. GSH fractional synthesis rate (FSR) was assessed from [15N]glutamate incorporation into blood and gut GSH, and duodenal protein synthesis from [13C]leucine incorporation into gut protein. Glutamine supplementation failed to alter erythrocyte GSH concentration (2189±86 vs. 1994±102 μmol L−1 for glutamine vs. control; ns) or FSR (64±17% vs. 74±20% day−1; ns). In the duodenum, glutamine supplementation was associated with a 92% rise in reduced/oxidized GSH ratio (P=.024) and with a 44% decline in GSH FSR (96±15% day−1 vs. 170±18% day−1; P=.005), whereas total GSH concentration remained unchanged (808±154 vs. 740±127 μmol kg−1; P=.779). We conclude that, in dogs receiving enteral nutrition after a 3-day fast: (1) glutamine availability does not affect blood GSH, and, (2) in contrast, in the duodenum, the preserved GSH pool, along with a decreased synthesis rate, suggests that glutamine may maintain GSH pool and intestinal redox status by acutely decreasing GSH utilization. [Copyright &y& Elsevier]
Details
- Language :
- English
- ISSN :
- 09552863
- Volume :
- 18
- Issue :
- 1
- Database :
- Academic Search Index
- Journal :
- Journal of Nutritional Biochemistry
- Publication Type :
- Academic Journal
- Accession number :
- 23280169
- Full Text :
- https://doi.org/10.1016/j.jnutbio.2006.02.002