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Involvement of a Xenopus nuclear GTP-binding protein in optic primordia formation.
- Source :
-
Development, Growth & Differentiation . Dec2006, Vol. 48 Issue 9, p575-585. 11p. - Publication Year :
- 2006
-
Abstract
- Using a subtracted Xenopus cDNA library based on the differential sensitivity of anterior and posterior genes to retinoic acid, we isolated a novel Xenopus nuclear GTP-binding protein (XGB). XGB is expressed prominently in the optic primordia at the tailbud stage. The N-terminal region of XGB contains a set of GTP-binding protein motifs, and the C-terminal region contains two putative nuclear localization signals and two coiled regions. A GFP-XGB fusion protein was expressed in the nucleus of NIH3T3 cells where it bound to subnuclear structures. Truncated C-terminal constructs of XGB containing both nuclear localization signal(s) and coiled region(s) suppressed eye formation, whereas neither the N-terminal construct nor constructs with a mutated GTP-binding protein motif affected eye formation. Expression of Pax6 and Rx1 genes, which are crucial for eye development, was reduced in embryos overexpressing the C-terminal constructs of XGB. Suppression of Pax6 and Rx1 at earlier developmental stages as well as perturbation of eye formation at later stages was counteracted by co-expression of wild-type XGB. We conclude that XGB plays a role in the formation of optic primordia through activation of at least two eye field transcription factors. [ABSTRACT FROM AUTHOR]
Details
- Language :
- English
- ISSN :
- 00121592
- Volume :
- 48
- Issue :
- 9
- Database :
- Academic Search Index
- Journal :
- Development, Growth & Differentiation
- Publication Type :
- Academic Journal
- Accession number :
- 23017759
- Full Text :
- https://doi.org/10.1111/j.1440-169X.2006.00891.x