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Synthesis of minus-strand copies of a viral transgene during viral infections of transgenic plants
- Source :
-
Virus Research . Dec2006, Issue 1/2, p171-174. 4p. - Publication Year :
- 2006
-
Abstract
- Abstract: Viral transgenes designed to provide resistance to specific plant viruses frequently consist of the coat protein gene and a contiguous 3′ untranslated region (3′UTR) of viral origin. In many RNA viruses the viral 3′UTR establishes a recognition and initiation site for viral RNA replication. Thus the transgenic transcript may contain a functional virus replication site. Experiments were designed to determine if a challenging virus would recognize this replication site on a nuclear derived transcript and synthesize the complementary RNA. These data demonstrate that upon infection by a virus that recognizes the viral replication site, a full-length complement of the transgenic transcript is produced. In these experiments the replication complex of Brome Mosaic bromovirus recognized the transgenic transcript derived from a Cowpea Chlorotic Mottle bromovirus transgene. The resulting RNA may contribute to RNA recombination events. [Copyright &y& Elsevier]
- Subjects :
- *TRANSGENIC organisms
*RNA
*VIRUS diseases
*VIRAL replication
Subjects
Details
- Language :
- English
- ISSN :
- 01681702
- Issue :
- 1/2
- Database :
- Academic Search Index
- Journal :
- Virus Research
- Publication Type :
- Academic Journal
- Accession number :
- 22936170
- Full Text :
- https://doi.org/10.1016/j.virusres.2006.05.009