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Multiple regulatory regions and tissue-specific transcription initiation mediate the expression of NEMO/IKKγ gene
- Source :
-
Gene . Nov2006, Vol. 383, p99-107. 9p. - Publication Year :
- 2006
-
Abstract
- Abstract: NEMO/IKKγ gene, which is responsible of two allelic diseases in human, EDA-ID and IP, encodes for a protein with a central regulatory role in the activation of the NF-kB pathway. We here provide insights into the molecular mechanism governing NEMO/IKKγ expression. We mapped 4 distinctive NEMO/IKKγ transcription start sites each corresponding to an alternative first exon, controlled by two conserved promoters. A distal promoter, named promoter A, located 10 kb upstream of the coding region and a proximal promoter, promoter B, with strong bi-directional activity driving also the transcription of G6PD gene in the opposite direction. The promoter B is housekeeping, it is embedded in a CpG island, required for proper expression and it is down-regulated by methylation. The promoter A is active in cells of hepatic origin and it directs transcription of the main NEMO/IKKγ 5′ UTR alternative transcript in liver, which starts at a tissue-specific site. Qualitative and quantitative expression analysis revealed that each NEMO/IKKγ 5′ UTR alternative transcript has different expression profiles indicating that the control of NEMO/IKKγ expression is mediated through tissue-specific transcription initiation sites and multiple regulatory regions. [Copyright &y& Elsevier]
- Subjects :
- *DYSPLASIA
*DNA polymerases
*CELL transformation
*BILIARY tract
Subjects
Details
- Language :
- English
- ISSN :
- 03781119
- Volume :
- 383
- Database :
- Academic Search Index
- Journal :
- Gene
- Publication Type :
- Academic Journal
- Accession number :
- 22826884
- Full Text :
- https://doi.org/10.1016/j.gene.2006.07.022