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Overexpression and Characterization of a Carboxypeptidase from the HyperthermophiIic Archaeon Thermococcus sp. NA1.

Authors :
Hyun Sook Lee
Yun Jae Kim
Seung Seob Bae
Jeong Ho Jeon
Jae Kyu Lim
Sung Gyun Kang
Jung-Hyun Lee
Source :
Bioscience, Biotechnology & Biochemistry. May2006, Vol. 70 Issue 5, p1140-1147. 8p. 2 Diagrams, 2 Charts, 4 Graphs.
Publication Year :
2006

Abstract

The article presents a study that describes the cloning of the gene and characterization of the recombinant enzyme with regard to its enzymatic properties. Escherichia coli was used for plasmid propagation and nucleotide sequencing. The carboxypeptidase gene was cloned and overexpressed in Escherichia coli.

Details

Language :
English
ISSN :
09168451
Volume :
70
Issue :
5
Database :
Academic Search Index
Journal :
Bioscience, Biotechnology & Biochemistry
Publication Type :
Academic Journal
Accession number :
22090861
Full Text :
https://doi.org/10.1271/bbb.70.1140