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Vaccination with Irradiated Listeria Induces Protective T Cell Immunity
- Source :
-
Immunity (10747613) . Jul2006, Vol. 25 Issue 1, p143-152. 10p. - Publication Year :
- 2006
-
Abstract
- Summary: We evaluated γ-irradiated Listeria monocytogenes as a killed bacterial vaccine, testing the hypothesis that irradiation preserves antigenic and adjuvant structures destroyed by traditional heat or chemical inactivation. Irradiated Listeria monocytogenes (LM), unlike heat-killed LM, efficiently activated dendritic cells via Toll-like receptors and induced protective T cell responses in mice. Like live LM, irradiated LM induced Toll-like-receptor-independent T cell priming. Cross-presentation of irradiated listerial antigens to CD8+ T cells involved TAP- and proteasome-dependent cytosolic antigen processing. These results establish that killed LM can induce protective T cell responses, previously thought to require live infection. γ-irradiation may be potentially applied to numerous bacterial vaccine candidates, and irradiated bacteria could serve as a vaccine platform for recombinant antigens derived from other pathogens, allergens, or tumors. [Copyright &y& Elsevier]
- Subjects :
- *LISTERIA
*T cells
*GRAM-positive bacteria
*LYMPHOCYTES
Subjects
Details
- Language :
- English
- ISSN :
- 10747613
- Volume :
- 25
- Issue :
- 1
- Database :
- Academic Search Index
- Journal :
- Immunity (10747613)
- Publication Type :
- Academic Journal
- Accession number :
- 21666796
- Full Text :
- https://doi.org/10.1016/j.immuni.2006.05.013