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A burst of auxilin recruitment determines the onset of cIathrin-coated vesicle uncoating.

Authors :
Massol, Ramiro H.
Boll, Werner
Griffin, April M.
Kirchhausen, Tomas
Source :
Proceedings of the National Academy of Sciences of the United States of America. 7/5/2006, Vol. 103 Issue 27, p10265-10270. 6p. 5 Diagrams.
Publication Year :
2006

Abstract

Clathrin-coated pits assemble on a membrane and pinch off as coated vesicles. The released vesicles then rapidly lose their clathrin coats in a process mediated by the ATPase Hsc70, recruited by auxilin, a J-domain-containing cofactor. How is the uncoating process regulated? We find that during coat assembly small and variable amounts of auxilin are recruited transiently but that a much larger burst of association occurs after the peak of dynamin signal, during the transition between membrane constriction and vesicle budding. We show that the auxilin burst depends on domains of the protein likely to interact with lipid head groups. We conclude that the timing of auxilin recruitment determines the onset of uncoating. We propose that, when a diffusion barrier is established at the constricting neck of a fully formed coated pit and immediately after vesicle budding, accumulation of a specific lipid can recruit sufficient auxilin molecules to trigger uncoating. [ABSTRACT FROM AUTHOR]

Details

Language :
English
ISSN :
00278424
Volume :
103
Issue :
27
Database :
Academic Search Index
Journal :
Proceedings of the National Academy of Sciences of the United States of America
Publication Type :
Academic Journal
Accession number :
21653454
Full Text :
https://doi.org/10.1073/pnas.0603369103