Regulatory components at the csgD promoter – additional roles for OmpR and integration host factor and role of the 5′ untranslated region.

CsgD is a master regulator of multicellular behaviour in Salmonella enterica serovar Typhimurium. Expression of CsgD is highly regulated on the transcriptional level. A nucleo-protein complex had been defined where the global regulators OmpR and integration host factor (IHF) bind up- and downstream of the csgD core promoter. In this study, the nucleo-protein complex of P csgD was extended through characterization of additional OmpR and IHF binding sites that influence the transcriptional activity of the csgD promoter. Furthermore, the role of the 174 bp long 5′-untranslated region on transcriptional activity was defined. [ABSTRACT FROM AUTHOR]