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Translation of Semliki-Forest-Virus 42-S RNA in a Mouse Cell-Free System to give Virus-Coat Proteins.
- Source :
-
European Journal of Biochemistry . Nov74 Part 1, Vol. 49 Issue 1, p101-110. 10p. 4 Graphs. - Publication Year :
- 1974
-
Abstract
- Semliki-Forest-virus 42-S virion RNA is translated in a mouse ascites cell-free system. One of the major polypeptides made <em>in vitro</em> has been identified as Semliki-Forest-virus capsid protein by co-electrophoresis on dodecylsulfate -- polyacrylamide gels and by tryptic peptide analysis on paper. Another major product of the cell-free system has been characterised as envelope protein by peptide analysis but it does not co-electrophorese on gels with envelope proteins from purified virions. It is possible that the nascent, non-glycosylated polypeptide is partially degraded by proleolytic enzymes present in the cell-free extract, or alternatively that fragments of envelope protein are produced by premature termination on the viral RNA. Several other polypeptides are synthesised in the cell-free system in variable amounts but these have not been identified. The genetic content and the mechanism of translation of Semliki-Forest-virus 42-S RNA are discussed. [ABSTRACT FROM AUTHOR]
- Subjects :
- *SEMLIKI Forest virus
*RNA
*PROTEINS
*PEPTIDE hormones
*BIOCHEMISTRY
Subjects
Details
- Language :
- English
- ISSN :
- 00142956
- Volume :
- 49
- Issue :
- 1
- Database :
- Academic Search Index
- Journal :
- European Journal of Biochemistry
- Publication Type :
- Academic Journal
- Accession number :
- 21133924
- Full Text :
- https://doi.org/10.1111/j.1432-1033.1974.tb03815.x