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Response surface methodological approach for optimization of enzymatic synthesis of sorbitan methacrylate
- Source :
-
Enzyme & Microbial Technology . Jul2006, Vol. 39 Issue 3, p381-386. 6p. - Publication Year :
- 2006
-
Abstract
- Abstract: Sorbitan methacrylate was synthesized from sorbitan dehydrated from d-sorbitol using an immobilized lipase. To optimize the enzymatic synthesis of sorbitan methacrylate, response surface methodology was applied to determine the effects of five-level-four-factors and their reciprocal interactions on sorbitan methacrylate biosynthesis. A total of 30 individual experiments were performed, which were designed to study reaction temperature, reaction time, enzyme amount and substrate molar ratio. A statistical model predicted that the highest conversion yield of sorbitan methacrylate was 100%, at the following optimized reaction conditions: a reaction temperature of 43.06°C, a reaction time of 164.25min, an enzyme amount of 7.47%, and a substrate molar ratio of 3.98:1. Using these optimal factor values under experimental conditions in four independent replicates, the average conversion yield reached 98.7±1.2% and was well within the value predicted by the model. [Copyright &y& Elsevier]
- Subjects :
- *METHYL methacrylate
*ENZYMES
*POLYOLS
*ACRYLATES
Subjects
Details
- Language :
- English
- ISSN :
- 01410229
- Volume :
- 39
- Issue :
- 3
- Database :
- Academic Search Index
- Journal :
- Enzyme & Microbial Technology
- Publication Type :
- Academic Journal
- Accession number :
- 20962907
- Full Text :
- https://doi.org/10.1016/j.enzmictec.2005.11.046