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Studies on cleavage of DNA by N-phosphoryl branched peptides
- Source :
-
Peptides . Jun2006, Vol. 27 Issue 6, p1554-1560. 7p. - Publication Year :
- 2006
-
Abstract
- Abstract: It was found that N α,N ɛ-di[N-(O,O-diisopropyl)phosphoryl-l-leucy]-l-lysyl-methyl ester (1) and N α,N ɛ-di[N-(O,O-diisopropyl)phosphoryl-l-phenylalanyl]-l-lysyl-methyl ester (2) could cleave supercoiled DNA such as PUC19 efficiently in 40mM Britton-Robinson buffer. The cleavage activities for both were investigated by agarose gel electrophoresis. The T4 ligase experiments implied that the cleavage of DNA occurs via a hydrolytic path. The results showed that the cleavage reaction of DNA is dependent on the value of pH and ionic strength in the solution. DNA cleavage is more efficient by N-phosphoryl branched peptide 2 than by N-phosphoryl branched peptide 1. The experiments also show that hydrolysis of DNA by N-phosphoryl branched peptide 1 was accelerated in the presence of Mg2+ or Zn2+ ions. The interactions of DNA with N-phosphoryl branched peptides were also characterized by melting temperature measurements and circular dichroism (CD) techniques. On the basis of experimental data, the possible mechanism of interactions between DNA with N-phosphoryl branched peptides was discussed. [Copyright &y& Elsevier]
- Subjects :
- *DNA
*PEPTIDES
*DEOXYRIBOSE
*HYDROGEN-ion concentration
Subjects
Details
- Language :
- English
- ISSN :
- 01969781
- Volume :
- 27
- Issue :
- 6
- Database :
- Academic Search Index
- Journal :
- Peptides
- Publication Type :
- Academic Journal
- Accession number :
- 20958268
- Full Text :
- https://doi.org/10.1016/j.peptides.2005.11.020