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How lipid hydration and temperature affect the structure of DC-Chol–DOPE/DNA lipoplexes
- Source :
-
Chemical Physics Letters . May2006, Vol. 422 Issue 4-6, p439-445. 7p. - Publication Year :
- 2006
-
Abstract
- Abstract: Effect of lipid hydration on the structure of lamellar lipoplexes made of the cationic lipid 3-[N-(N,N-dimethylaminoethane)-carbamoyl]cholesterol (DC-Chol), the neutral ‘helper’ lipid dioleoylphosphatidylethanolamine (DOPE) and calf-thymus DNA was investigated by synchrotron small angle X-ray diffraction (SAXD). Here, we show that lipid hydration is the key factor regulating the equilibrium structure of lipoplexes. Thermotropic behavior was also investigated between 5 and 65°C. Both the membrane thickness and the water layer thickness were found to decrease linearly as a function of temperature while the one dimensional DNA rod lattice between lipid bilayers was found to enlarge. Structural results were interpreted in terms of recently proposed theoretical models. [Copyright &y& Elsevier]
- Subjects :
- *NUCLEIC acids
*HYDRATION
*ISOPENTENOIDS
*LOW-cholesterol diet
Subjects
Details
- Language :
- English
- ISSN :
- 00092614
- Volume :
- 422
- Issue :
- 4-6
- Database :
- Academic Search Index
- Journal :
- Chemical Physics Letters
- Publication Type :
- Academic Journal
- Accession number :
- 20748729
- Full Text :
- https://doi.org/10.1016/j.cplett.2006.02.106