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17β-Estradiol activates PI3K/Akt signaling pathway by estrogen receptor (ER)-dependent and ER-independent mechanisms in endometrial cancer cells
- Source :
-
Journal of Steroid Biochemistry & Molecular Biology . Apr2006, Vol. 99 Issue 1, p9-18. 10p. - Publication Year :
- 2006
-
Abstract
- Abstract: Cellular response to estrogen is mediated both by estrogen receptor (ER) binding to estrogen response element (ERE) and by non-nuclear actions like activation of signal transducing pathways. The main aims are to study if PI3K/Akt signaling pathway can be activated by 17β-estradiol (E2) via non-nuclear action and to investigate the relationship of the action of E2 and ER in endometrial cancer cells expressing with different status of ER. The levels of phosphorylated Akt (Ser473) (P-Akt) and total Akt were examined by western blot and Akt kinase activity was measured in cells after stimulation with 1μM E2 at different time points. Inhibitory role of LY294002 on activation of Akt induced by E2 and its estrogen antagonist, ICI182780 were also tested. P-Akt/Akt was used as a measure of activation of Akt. We found that maximum P-Akt/Akt and Akt kinase activity took place at 30min in Ishikawa cells and 15min in HEC-1A cells and the activation persisted for at least 2h after stimulation with 1μM E2. The activation of Akt elicited gradually with increasing doses of E2. PI3K inhibitor, LY294002, stopped the activating Akt in a dose-dependent manner and 50μM LY294002 completely blocked the activation of Akt induced by E2. ICI182780 could block the activation of PI3K/Akt in ER-positive Ishikawa cells but not in HEC-1A cells with poor-expressed ER. This study demonstrated that E2 is able to promptly activate PI3K/Akt signal pathway in Ishikawa cells in an ER-dependent manner and ER-independent in HEC-1A cells. Blockage of PI3K/Akt cascade may become a potential and effective way to control endometrial carcinoma, especially in ER-negative cancers, which show no response to endocrinal therapy. [Copyright &y& Elsevier]
- Subjects :
- *ESTRADIOL
*SELECTIVE estrogen receptor modulators
*MENSTRUAL cycle
*CANCER cells
Subjects
Details
- Language :
- English
- ISSN :
- 09600760
- Volume :
- 99
- Issue :
- 1
- Database :
- Academic Search Index
- Journal :
- Journal of Steroid Biochemistry & Molecular Biology
- Publication Type :
- Academic Journal
- Accession number :
- 20622369
- Full Text :
- https://doi.org/10.1016/j.jsbmb.2005.11.013