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Tumor suppressive efficacy through augmentation of tumor-infiltrating immune cells by intratumoral injection of chemokine-expressing adenoviral vector.
- Source :
-
Cancer Gene Therapy . Apr2006, Vol. 13 Issue 4, p393-405. 13p. 3 Color Photographs, 1 Black and White Photograph, 4 Graphs. - Publication Year :
- 2006
-
Abstract
- Our goal in the present study was to evaluate antitumor effects and frequency of tumor-infiltrating immune cells upon intratumoral injection of RGD fiber-mutant adenoviral vector (AdRGD) encoding the chemokines CCL17, CCL19, CCL20, CCL21, CCL22, CCL27, XCL1, and CX3CL1. Among eight kinds of chemokine-expressing AdRGDs, AdRGD-CCL19 injection most efficiently induced infiltration of T cells into established B16BL6 tumor parenchyma, whereas most of these T cells were perforin-negative in immunohistochemical analysis. Additionally, the growth of AdRGD-CCL19-injected tumors decreased only slightly as well as that of other tumors treated with each chemokine-expressing AdRGD, which indicated that accumulation of naive T cells in tumor tissue does not effectively damage the tumor cells. Tumor-bearing mice, in which B16BL6-specific T cells were elicited by dendritic cell-based immunization, demonstrated that intratumoral injection of AdRGD-CCL17, -CCL22, or -CCL27 could considerably suppress tumor growth and attract activated T cells. On the other hand, AdRGD-CCL19-injection in the immunized mice showed slight increase of tumor-infiltrating T cells compared to treatment using control vector. Collectively, although AdRGD-mediated chemokine gene transduction into established tumors would be very useful for augmentation of tumor-infiltrating immune cells, a combinational treatment that can systemically induce tumor-specific effector T cells is necessary for satisfactory antitumor efficacy.Cancer Gene Therapy (2006) 13, 393–405. doi:10.1038/sj.cgt.7700903; published online 14 October 2005 [ABSTRACT FROM AUTHOR]
Details
- Language :
- English
- ISSN :
- 09291903
- Volume :
- 13
- Issue :
- 4
- Database :
- Academic Search Index
- Journal :
- Cancer Gene Therapy
- Publication Type :
- Academic Journal
- Accession number :
- 20179532
- Full Text :
- https://doi.org/10.1038/sj.cgt.7700903