Electrochemical study on the behavior of Morin and its interaction with DNA

Abstract: Voltammetric behavior of Morin was studied in 0.1M HAc–NaAc+50mM KCl (pH 3.4) solution at glassy carbon electrode (GCE) using cyclic voltammetry (CV). Morin showed an irreversible anodic peak at 0.720V in CV which was involving two electrons and two protons. Also, the interaction of Morin with double-stranded calf thymus DNA (ctDNA) was studied by CV at GCE with an irreversible electrochemical equation. As a result of reaction with ctDNA, the voltammetric peak of Morin was a position shift and the peak current decreased. The diffusion coefficients of both free and binding Morin (D f =1.1086×10−7 cm2 s−1 and D b =8.2544×10−9 cm2 s−1), binding constant (K =1.7765×107 cm3 mol−1), and binding site size (s =0.8510) of the Morin–DNA complex were obtained simultaneously by non-linear fit analysis. The results demonstrate that Morin can bind to ctDNA in 0.1M HAc–NaAc+50mM KCl (pH 3.4) solution and the ring B of Morin intercalates between the DNA base pairs. [Copyright &y& Elsevier]