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Expression of cyclic nucleotide phosphodiesterase 3A in isolated rat submandibular acini
- Source :
-
Archives of Oral Biology . Feb2006, Vol. 51 Issue 2, p83-88. 6p. - Publication Year :
- 2006
-
Abstract
- Summary: Phosphodiesterase (PDE) 3 has been characterized in isolated rat submandibular acini. PDE3 activity was detected in homogenates of isolated rat submandibular acini; little or no PDE3 activity was found in ducts. About 62% of PDE3 activity in the acini was recovered in the supernatant fractions; 38% in particulate fractions. In the acini, but not ducts, PDE3A mRNA was detected by reverse transcriptase-polymerase chain reaction (RT-PCR). The PDE3-specific inhibitor, cilostamide, increased the ratio of apomucin mRNA/18s rRNA, as quantified by real-time RT-PCR. Our results indicate that PDE3A may be important in regulating cAMP pools that control acini functions. [Copyright &y& Elsevier]
- Subjects :
- *PHOSPHODIESTERASES
*SUBMANDIBULAR gland
*MESSENGER RNA
*POLYMERASE chain reaction
Subjects
Details
- Language :
- English
- ISSN :
- 00039969
- Volume :
- 51
- Issue :
- 2
- Database :
- Academic Search Index
- Journal :
- Archives of Oral Biology
- Publication Type :
- Academic Journal
- Accession number :
- 19396672
- Full Text :
- https://doi.org/10.1016/j.archoralbio.2005.06.012