Glycoform Analysis of Japanese Cedar Pollen Allergen, Cry j 1.

The article investigates the glycoform of the pollen allergen to find whether the Lewis a epitopes occur in the N-gylcan moiety of the Japanese cedar pollen glycollergen, Cry j 1. The N-gylcans were liberated from the cedar pollen glycoallergen by hydrazinolysis. The resulting sugar chains were N-acetylated and then coupled with 2-aminopyridine.