Removal of lysed blastomeres from frozen–thawed embryos improves implantation and pregnancy rates in frozen embryo transfer cycles

Objective: To evaluate the effect of degenerated (lysed) blastomere removal on implantation and pregnancy rates in cleavage-stage cryo–embryo transfer (ET) cycles. Design: Randomized clinical trial. Setting: Private reproductive medical center. Patient(s): A total of 88 patients who received frozen–thawed ET, divided into two groups. Intervention(s): Embryo freezing and thawing; opening of the zona pellucida and removal of cryodamaged blastomeres (in the study group), followed by same-day ET. Main Outcome Measure(s): Extent of survival of cleavage-stage embryos after the freeze–thaw procedure; embryo implantation and clinical pregnancies. Result(s): Oocyte number per patient, fertilization rate, embryo development rate (and quality), and freezing rates were similar in the two groups in the fresh cycle. In the control group, a total of 55 embryos (25%) of the 217 thawed remained fully intact, and 53 (26%) of the 207 in the study group remained intact. The average number of embryos transferred per group was similar (control, 3.4 ± 0.9; study, 3.3 ± 0.9). Implantation rates were 12% and 26% in the control and study groups, respectively. The clinical pregnancy rate was 23% in the control group and 64% in the study group when lysed cell removal was performed. Conclusion(s): The results show that pregnancy and implantation rates are higher in the study group; therefore, the removal of degenerated blastomeres may be beneficial to all patients who undergo cleavage-stage, frozen–thawed ET. [Copyright &y& Elsevier]