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Neutrophil activation induced by the lectin KM+ involves binding to CXCR2
- Source :
-
BBA - General Subjects . Jan2006, Vol. 1760 Issue 1, p86-94. 9p. - Publication Year :
- 2006
-
Abstract
- Abstract: The lectin KM+ from Artocarpus integrifolia, also known as artocarpin, induces neutrophil migration by haptotaxis. The interactions of KM+ with both neutrophils and the extracellular matrix depend on the lectin''s ability to recognize mannose-containing glycans. In the present study, we characterized the binding of KM+ to human neutrophils and the responses stimulated by this binding. Exposure to KM+ results in cell polarization, formation of a lamellipodium, and induction of deep ruffles on the cell surface. By fluorescence microscopy, we observed that KM+ is distributed homogeneously over the cell surface. KM+/ligand complexes are rapidly internalized, reaching maximum intracellular concentrations at 120 min, and decreasing thereafter. Furthermore, KM+ binding to the surface of human neutrophils is inhibited by the specific sugars, d-mannose or mannotriose. KM+-induced neutrophil migration is inhibited by pertussis toxin as well as by inhibition of CXCR2 activity. These results suggest that the KM+ ligand on the neutrophil surface is a G protein-coupled receptor (GPCR). The results also suggest that neutrophil migration induced by KM+ involves binding to CXCR2. [Copyright &y& Elsevier]
- Subjects :
- *LECTINS
*NEUTROPHILS
*GRANULOCYTES
*CONNECTIVE tissues
Subjects
Details
- Language :
- English
- ISSN :
- 03044165
- Volume :
- 1760
- Issue :
- 1
- Database :
- Academic Search Index
- Journal :
- BBA - General Subjects
- Publication Type :
- Academic Journal
- Accession number :
- 19199954
- Full Text :
- https://doi.org/10.1016/j.bbagen.2005.09.011