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Ciliary neurotrophic factor promotes inactivation of muscle Ca2+ channels via PKC
- Source :
-
Biochemical & Biophysical Research Communications . Dec2005, Vol. 338 Issue 3, p1572-1577. 6p. - Publication Year :
- 2005
-
Abstract
- Abstract: The actions of the ciliary neurotrophic factor (CNTF) were assessed on adult mouse skeletal muscle L-type Ca2+ currents and on Ca2+ release from sarcoplasmic reticulum. Currents were measured with the whole cell patch clamp technique. Ca2+ signals in response to single action potentials were recorded with Fluo3-AM. CNTF (20ng/ml) reversibly reduced the amplitude of Ca2+ channel currents by 50% within 15min. In addition, CNTF greatly increased the rate of inactivation during depolarizing pulses and shifted the steady state inactivation curve by −12mV. The effects of CNTF were mimicked by the PKC activator PMA and prevented by the PKC-inhibitor chelerythrine. In contrast to the effects on the Ca2+ conductance, charge movement and Ca2+ signals remained unaffected by CNTF. These results suggest that CNTF can rapidly decrease muscle Ca2+ channel currents by promoting inactivation, probably through an intracellular PKC-dependent mechanism. [Copyright &y& Elsevier]
- Subjects :
- *SARCOPLASMIC reticulum
*ORGANELLES
*MUSCLE cells
*CELLS
Subjects
Details
- Language :
- English
- ISSN :
- 0006291X
- Volume :
- 338
- Issue :
- 3
- Database :
- Academic Search Index
- Journal :
- Biochemical & Biophysical Research Communications
- Publication Type :
- Academic Journal
- Accession number :
- 19045747
- Full Text :
- https://doi.org/10.1016/j.bbrc.2005.10.125