Disruption of rpmJ Encoding Ribosomal Protein L36 Decreases the Expression of secY Upstream of the spc Operon and Inhibits Protein Translocation in Escherichia coli.

Examines the development of rpmJ gene null strains, AY101 and AY201, by replacing rpmJ with tetA, which encodes a membrane protein, and cat, which encodes a cytoplasmic chloramphenicol acetyltransferase, respectively. Decrease in the level of SecY mRNA in both disruptants; Correction of the phenotypes caused by the rpmJ null mutation by a plasmid carrying SecY; Roles of RpmJ in protein synthesis and translocation.