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Novel type aquaporin SIPs are mainly localized to the ER membrane and show cell-specific expression in Arabidopsis thaliana

Authors :
Ishikawa, Fumiyoshi
Suga, Shinobu
Uemura, Tomohiro
Sato, Masa H.
Maeshima, Masayoshi
Source :
FEBS Letters. Oct2005, Vol. 579 Issue 25, p5814-5820. 7p.
Publication Year :
2005

Abstract

Abstract: We investigated the fourth subgroup of Arabidopsis aquaporin, small and basic intrinsic proteins (SIPs). When they were expressed in yeast, SIP1;1 and SIP1;2, but not SIP2;1, gave water-channel activity. The transient expression of SIPs linked with green fluorescent protein in Arabidopsis cells and the subcellular fractionation of the tissue homogenate showed their ER localization. The SIP proteins were detected in all of the tissues, except for dry seeds. Histochemical analysis of promoter-β-glucuronidase fusions revealed the cell-specific expression of SIPs. SIP1;1 and SIP1;2 may function as water channels in the ER, while SIP2;1 might act as an ER channel for other small molecules or ions. [Copyright &y& Elsevier]

Details

Language :
English
ISSN :
00145793
Volume :
579
Issue :
25
Database :
Academic Search Index
Journal :
FEBS Letters
Publication Type :
Academic Journal
Accession number :
18782026
Full Text :
https://doi.org/10.1016/j.febslet.2005.09.076