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MDC1 interacts with Rad51 and facilitates homologous recombination.
- Source :
-
Nature Structural & Molecular Biology . Oct2005, Vol. 12 Issue 10, p902-909. 8p. 2 Color Photographs, 3 Diagrams, 6 Graphs. - Publication Year :
- 2005
-
Abstract
- Mediator of DNA damage checkpoint protein-1 (MDC1) is a recently identified nuclear protein that participates in DNA-damage sensing and signaling. Here we report that knockdown of MDC1 by RNA interference results in cellular hypersensitivity to the DNA cross-linking agent mitomycin C and ionizing radiation and in impaired homology-mediated repair of double-strand breaks in DNA. MDC1 forms a complex with Rad51 through a direct interaction with the forkhead-associated domain of MDC1, not the BRCA1 C-terminal domain. Depletion of MDC1 results in impaired formation of Rad51 ionizing radiation–induced foci, reduced amounts of nuclear and chromatin-bound Rad51, and a corresponding increase in Rad51 protein degradation. Together, our findings suggest that MDC1 functions in Rad51-mediated homologous recombination by retaining Rad51 in chromatin. [ABSTRACT FROM AUTHOR]
- Subjects :
- *DNA-binding proteins
*PROTEINS
*DNA damage
*RNA
*CHROMATIN
*PROTEOMICS
Subjects
Details
- Language :
- English
- ISSN :
- 15459993
- Volume :
- 12
- Issue :
- 10
- Database :
- Academic Search Index
- Journal :
- Nature Structural & Molecular Biology
- Publication Type :
- Academic Journal
- Accession number :
- 18458989
- Full Text :
- https://doi.org/10.1038/nsmb991