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ISOLATION AND CHARACTERIZATION OF CALPAINS FROM RAINBOW TROUT MUSCLE AND THEIR ROLE IN TEXTURE DEVELOPMENT.

ISOLATION AND CHARACTERIZATION OF CALPAINS FROM RAINBOW TROUT MUSCLE AND THEIR ROLE IN TEXTURE DEVELOPMENT.

Authors :
Salem, M.
Kenney, P. B.
Killefer, J.
Nath, J.
Source :
Journal of Muscle Foods. Oct2004, Vol. 15 Issue 4, p245-255. 11p. 3 Graphs.
Publication Year :
2004

Abstract

A single anion-exchange column was used to resolve calpain I and II and their endogenous inhibitor, calpastatin, from the white muscle of rainbow trout (Oncorhynchus mykiss ). Calcium requirement assays showed that calpain I and II required approximately 0.2 mM and 2.5 mM, respectively, for half maximal activities. Isolation of calpain I and calpastatin were confirmed by Western blot. Incubation of trout muscle for 12 h postmortem, in a buffer containing CaCl2, decreased (P < 0.0001) shear force compared to samples incubated in a buffer containing EDTA. This effect indicates that softening of fish muscle can be accelerated by activation of calpains with exogenous calcium. [ABSTRACT FROM AUTHOR]

Details

Language :
English
ISSN :
10460756
Volume :
15
Issue :
4
Database :
Academic Search Index
Journal :
Journal of Muscle Foods
Publication Type :
Academic Journal
Accession number :
18354263
Full Text :
https://doi.org/10.1111/j.1745-4573.2004.06803.x