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ISOLATION AND CHARACTERIZATION OF CALPAINS FROM RAINBOW TROUT MUSCLE AND THEIR ROLE IN TEXTURE DEVELOPMENT.
ISOLATION AND CHARACTERIZATION OF CALPAINS FROM RAINBOW TROUT MUSCLE AND THEIR ROLE IN TEXTURE DEVELOPMENT.
- Source :
-
Journal of Muscle Foods . Oct2004, Vol. 15 Issue 4, p245-255. 11p. 3 Graphs. - Publication Year :
- 2004
-
Abstract
- A single anion-exchange column was used to resolve calpain I and II and their endogenous inhibitor, calpastatin, from the white muscle of rainbow trout (Oncorhynchus mykiss ). Calcium requirement assays showed that calpain I and II required approximately 0.2 mM and 2.5 mM, respectively, for half maximal activities. Isolation of calpain I and calpastatin were confirmed by Western blot. Incubation of trout muscle for 12 h postmortem, in a buffer containing CaCl2, decreased (P < 0.0001) shear force compared to samples incubated in a buffer containing EDTA. This effect indicates that softening of fish muscle can be accelerated by activation of calpains with exogenous calcium. [ABSTRACT FROM AUTHOR]
- Subjects :
- *SALMONIDAE
*TROUT
*FISHES
*FOOD
*COOKING
Subjects
Details
- Language :
- English
- ISSN :
- 10460756
- Volume :
- 15
- Issue :
- 4
- Database :
- Academic Search Index
- Journal :
- Journal of Muscle Foods
- Publication Type :
- Academic Journal
- Accession number :
- 18354263
- Full Text :
- https://doi.org/10.1111/j.1745-4573.2004.06803.x