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Role of conserved cysteines in mediating sulfur transfer from IscS to IscU
- Source :
-
FEBS Letters . Sep2005, Vol. 579 Issue 23, p5236-5240. 5p. - Publication Year :
- 2005
-
Abstract
- Abstract: The role of the three conserved cysteine residues on Azotobacter vinelandii IscU in accepting sulfane sulfur and forming a covalent complex with IscS has been evaluated using electrospray-ionization mass spectrometry studies of variants involving individual cysteine-to-alanine substitutions. The results reveal that IscS can transfer sulfur to each of the three alanine-substituted forms of IscU to yield persulfide or polysulfide species, and formation of a heterodisulfide covalent complex between IscS and Cys37 on IscU. It is concluded that S transfer from IscS to IscU does not involve a specific cysteine on IscU or the formation of an IscS–IscU heterodisulfide complex. [Copyright &y& Elsevier]
- Subjects :
- *CYSTEINE proteinases
*SPECTRUM analysis
*NITROGEN fixation
*BIOGEOCHEMICAL cycles
Subjects
Details
- Language :
- English
- ISSN :
- 00145793
- Volume :
- 579
- Issue :
- 23
- Database :
- Academic Search Index
- Journal :
- FEBS Letters
- Publication Type :
- Academic Journal
- Accession number :
- 18344178
- Full Text :
- https://doi.org/10.1016/j.febslet.2005.08.046