Back to Search
Start Over
BACH1 is critical for homologous recombination and appears to be the Fanconi anemia gene product FANCJ
- Source :
-
Cancer Cell . Sep2005, Vol. 8 Issue 3, p255-265. 11p. - Publication Year :
- 2005
-
Abstract
- Summary: We showed in this study that cells deficient of the BRCA1-associated BACH1 helicase, also known as BRIP1, failed to elicit homologous recombination (HR) after DNA double-stranded breaks (DSBs). BACH1-deficient cells were also sensitive to mitomycin C (MMC) and underwent MMC-induced chromosome instability. Moreover, we identified a homozygous nonsense mutation in BACH1 in a FA-J patient-derived cell line and could not detect BACH1 protein in this cell line. Expression of wild-type BACH1 in this cell line reduced the accumulation of cells at G2/M phases following exposure to DNA crosslinkers, a characteristic of Fanconi anemia (FA) cells. These results support the conclusion that BACH1 is FANCJ. [Copyright &y& Elsevier]
- Subjects :
- *DNA helicases
*CELL lines
*CELLS
*DNA
*GENETIC mutation
Subjects
Details
- Language :
- English
- ISSN :
- 15356108
- Volume :
- 8
- Issue :
- 3
- Database :
- Academic Search Index
- Journal :
- Cancer Cell
- Publication Type :
- Academic Journal
- Accession number :
- 18305727
- Full Text :
- https://doi.org/10.1016/j.ccr.2005.08.004