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Huperzine A protects SHSY5Y neuroblastoma cells against oxidative stress damage via nerve growth factor production
- Source :
-
European Journal of Pharmacology . Sep2005, Vol. 519 Issue 1/2, p9-15. 7p. - Publication Year :
- 2005
-
Abstract
- Abstract: Our previous study demonstrated that huperzine A, a selective acetylcholinesterase inhibitor, stimulates the synthesis of nerve growth factor (NGF) in cultured rat cortical astrocytes. The present studies are designed to examine if huperzine A exerts its neuroprotective activity against oxidative stress damage through increasing the synthesis of NGF in SHSY5Y neuroblastoma cells. Transient exposure of the cells to 200 μM H2O2 triggered a significant reduction of cell viability and decreased the mRNA and protein levels of NGF, neurotrophin receptor P75 (P75NTR) receptor and tyrosine kinase A (TrkA) receptor. Incubation of cells with 10 μM huperzine A prior to H2O2 exposure significantly elevated their survival and restored the mRNA and protein levels of NGF, P75NTR receptor and TrkA receptor. These neuroprotective effects of huperzine A on H2O2-induced cytotoxicity were blocked by the TrkA receptor phosphorylation inhibitor K252α, and were antagonized by the mitogen-activated protein (MAP)/extracellular signal-regulated kinase (ERK) inhibitor, PD98059. The present results indicate that the cytoprotective effect of huperzine A is mediated at least partly by up-regulated NGF and NGF receptors. The results also show that the MAP/ERK kinase signal pathway is crucial for huperzine A to protect against H2O2-induced damage in SHSY5Y cells. [Copyright &y& Elsevier]
- Subjects :
- *NEUROBLASTOMA
*TYROSINE
*PROTEIN-tyrosine kinases
*CYTOKINES
Subjects
Details
- Language :
- English
- ISSN :
- 00142999
- Volume :
- 519
- Issue :
- 1/2
- Database :
- Academic Search Index
- Journal :
- European Journal of Pharmacology
- Publication Type :
- Academic Journal
- Accession number :
- 18263508
- Full Text :
- https://doi.org/10.1016/j.ejphar.2005.06.026