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Cyclin D3 interacts with vitamin D receptor and regulates its transcription activity
- Source :
-
Biochemical & Biophysical Research Communications . Sep2005, Vol. 335 Issue 3, p739-748. 10p. - Publication Year :
- 2005
-
Abstract
- Abstract: D-type cyclins are essential for the progression through the G1 phase of the cell cycle. Besides serving as cell cycle regulators, D-type cyclins were recently reported to have transcription regulation functions. Here, we report that cyclin D3 is a new interacting partner of vitamin D receptor (VDR), a member of the superfamily of nuclear receptors for steroid hormones, thyroid hormone, and the fat-soluble vitamins A and D. The interaction was confirmed with methods of yeast two-hybrid system, in vitro binding analysis and in vivo co-immunoprecipitation. Cyclin D3 interacted with VDR in a ligand-independent manner, but treatment of the ligand, 1,25-dihydroxyvitamin D3, strengthened the interaction. Confocal microscopy analysis showed that ligand-activated VDR led to an accumulation of cyclin D3 in the nuclear region. Cyclin D3 up-regulated transcriptional activity of VDR and this effect was counteracted by overexpression of CDK4 and CDK6. These findings provide us a new clue to understand the transcription regulation functions of D-type cyclins. [Copyright &y& Elsevier]
- Subjects :
- *CYCLINS
*VITAMIN D
*GENETIC transcription
*GROWTH factors
Subjects
Details
- Language :
- English
- ISSN :
- 0006291X
- Volume :
- 335
- Issue :
- 3
- Database :
- Academic Search Index
- Journal :
- Biochemical & Biophysical Research Communications
- Publication Type :
- Academic Journal
- Accession number :
- 18241828
- Full Text :
- https://doi.org/10.1016/j.bbrc.2005.07.141