柚果多糖对斜带石斑鱼 脂肪沉积和肝脏健康的影响.

The experiment aimed to investigate the effects of pomelo fruit polysaccharide on reducing fat deposition and improving liver health in Epinephelus coioides. Selected Epinephelus coioides with a body weight of about 11 g, divided into five groups. There were three replicates in each group: Control group (fat content 11.06%), low-fat group (fat content 7.49%), low-fat + pomelo fruit polysaccharide group (low-fat diet +400 mg/kg pomelo fruit polysaccharide), high-fat group (fat content 15.32%) and high-fat + pomelo fruit polysaccharide group (high-fat diet +400 mg/kg pomelo fruit polysaccharide). 20 fish per repeat. The pre-test period was two weeks, the formal test period was eight weeks. The results showed that the weight gain rate (WGR) and specific growth rate (SGR) of Epinephelus coioides in the low-fat group were significantly lower than those in the control group (P<0.05). The low-fat + pomelo fruit polysaccharide group and the high-fat + pomelo fruit polysaccharide group were higher than the low-fat group and the high-fat group, respectively, but without significant differences (P>0.05). Compared to the high-fat group, the whole fish and muscle crude fat content and hepatosomatic index (HSI) in the high-fat + pomelo fruit polysaccharide group were significantly reduced (P<0.05). The low-fat group and high-fat group showed liver damage, but the low-fat + pomelo fruit polysaccharide group and the high-fat + pomelo fruit polysaccharide group exhibited significant improvements in liver tissue structure damage, and the liver lipid droplet content in the high-fat + pomelo fruit polysaccharide group was significantly lower than that in the high-fat group. The high-fat + pomelo fruit polysaccharide group significantly inhibited the upregulation of inflammation and apoptosis-related genes interleukin-1β (IL-1β), interleukin-8 (IL-8), caspase protease-3 (caspase-3), and caspase protease-8 (caspase-8). The study indicates that the addition of pomelo polysaccharide in low fat and high fat diets had no effect on the growth performance of Epinephelus coioides, but it could reduce the increase of crude fat content in whole fish and muscle and fat deposition in liver caused by high fat diet, enhance immune function and improve liver health. [ABSTRACT FROM AUTHOR]