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Construction of transgenic Bacillus mucilaginosus strain with improved phytase secretion.
- Source :
-
Journal of Applied Microbiology . Oct2005, Vol. 99 Issue 4, p878-884. 7p. - Publication Year :
- 2005
-
Abstract
- Aims: To construct a transgenic Bacillus mucilaginosus strain to increase the secretion capability of a wild-type isolate of B. mucilaginosus D4B1 to hydrolyse phytate phosphorus, which can be used as a microbial fertilizer in field application. Methods and Results: We constructed a phytase secreting expression vector pSP43 with a mini-Tn5 transposon and a Aspergillus fumigatus phytase expression cassette. The vector pSP43 was successfully transferred into the wild-type B. mucilaginosus using the particle bombardment method, and three transgenic strains with a stable copy of phytase expression cassette integrated into the chromosome of the B. mucilaginosus by Tn5 transposition were selected. The phytase activity of the engineered strains increased 36–46-fold when compared with the wild-type strain of D4B1. Conclusions: The A. fumigatus phytase gene can be expressed under the direction of p43 promoter in B. mucilaginosus. The expression protein is secreted extracellularly and newly constructed strains showed a high phytase activity. Significance and Impact of the Study: A transgenic Bacillus strain by the particle bombardment method was constructed. [ABSTRACT FROM AUTHOR]
- Subjects :
- *BACILLUS (Bacteria)
*PHOSPHORUS
*PHYTASES
*TRANSPOSONS
*MOBILE genetic elements
Subjects
Details
- Language :
- English
- ISSN :
- 13645072
- Volume :
- 99
- Issue :
- 4
- Database :
- Academic Search Index
- Journal :
- Journal of Applied Microbiology
- Publication Type :
- Academic Journal
- Accession number :
- 18220906
- Full Text :
- https://doi.org/10.1111/j.1365-2672.2005.02683.x