干旱胁迫对葡萄生理特性和叶片胁迫相关基因表达的影响.

[Objectives] The paper aimed to study the effects of drought stress on the physiological characteristics of grapes and the expression of stress-related genes. [Methods] The stress symptoms of the plants of ‘Shine Muscat' and ‘Kyoho' grape varieties, serving as test materials, were investigated through artificial drought stress simulation methods. Physiological indicators were measured, and principal component analysis and membership function analysis were conducted on these indicators for the two varieties under drought stress treatment. Furthermore, the expression levels of stress-related genes in the leaves were determined. [Results] The drought tolerance symptoms of both ‘Kyoho' and ‘Shine Muscat' were characterized by leaf wilting with leaf margin scorching at the base of the plant in the early period of drought stress, and green wilting and yellowing at the end of the stress period, but ‘Shine Muscat' showed drought stress symptoms earlier than those of ‘Kyoho'. With the prolongation of drought stress time, the leaf drought index of two grape varieties increased, the plant height and stem thickness showed a trend of increasing and then decreasing, the chlorophyll content declined, the activities of the peroxidase, superoxide dismutase, and ascorbate peroxidase showed a trend of increasing and then decreasing, and the quantum yield of the non-regulated energy dissipation in the PSⅡ, malondialdehyde content, proline content and soluble protein content showed an increasing trend. The average affiliation function values of ‘Kyoho' and ‘Shine Muscat' under drought stress were 0.47 and 0.46 respectively, and ‘Kyoho' was slightly more tolerant to drought stress than ‘Shine Muscat'. The RT-qPCR results showed that drought stress treatment significantly up-regulated the expression levels of the genes of VvPIP1, VvWRKY71, VvPP2C2 and VvRD22 in the leaves of two grape varieties. [Conclusions] Under drought stress, the response of ‘Shine Muscat' and ‘Kyoho' grape to drought stress was the same but the degree of response differed, and the drought tolerance of ‘Kyoho' was higher than that of ‘Shine Muscat'. [ABSTRACT FROM AUTHOR]