Nicotinic receptors regulate B lymphocyte activation and immune response

Abstract: The presence of nicotinic acetylcholine receptors (nicotinic receptors) composed of either α7 or α4 and β2 subunits is revealed in B lymphocytes by means of radioligand binding assay and Cell ELISA. Mouse B lymphocytes contained 12,200±3200 of epibatidine-binding sites and 3130±750 of α-Bungarotoxin-binding sites per cell. Mice lacking nicotinic receptor subunits α4, β2 or α7 had less serum IgG and IgG-producing cells in the spleen, but showed stronger immune response to both protein antigen in vivo and CD40-specific antibody in vitro than wild-type mice. Anti-CD40-stimulated proliferation of B lymphocytes from β2 knockout, but not wild-type mice was inhibited with nicotine. Our results indicate that signalling through nicotinic receptors affects both the pre-immune state and activation of B lymphocytes in the immune response, possibly via CD40-dependent pathway. This could contribute to immune depression found in tobacco smokers. [Copyright &y& Elsevier]