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Molecular Diagnosis of Helicobacter pylori Endosymbiont in Acanthamoeba-Positive Samples in Laboratory Conditions and in the Hospital Environments.

Authors :
Mohammadi, Alireza
Dalimi, Abdolhossein
Ghafarifar, Fatemeh
Pirestani, Majid
Akbari, Majid
Source :
Iranian Journal of Parasitology. Oct-Dec2024, Vol. 19 Issue 4, p397-407. 11p.
Publication Year :
2024

Abstract

Background: We aimed to identity Helicobacter pylori endosymbiont in Acanthamoeba-positive samples in natural and laboratory conditions. Methods: Overall, 134 samples were collected from hospital environments. Microscopic and PCR test were used for detection of Acanthamoeba and H. pylori. The real-time PCR method was used to check the active presence of H. pylori within Acanthamoeba under natural conditions from hospital samples and in coculture laboratory conditions. Results: The rate of contamination of hospital samples with Acanthamoeba was 44.7%. Out of 42 Acanthamoeba PCR-positive samples, 13 isolates (31%) were positive in terms of H. pylori endosymbiont according to sampling location. H. pylori is able to penetrate and enter the Acanthamoeba parasite. Conclusion: H. pylori is able to contaminate Acanthamoeba in natural and laboratory conditions. The presence of pathogenic Acanthamoeba in various hospital environments and the hiding of Helicobacter as an endosymbiont inside it can pose a serious threat to the health of hospitalized patients. [ABSTRACT FROM AUTHOR]

Details

Language :
English
ISSN :
17357020
Volume :
19
Issue :
4
Database :
Academic Search Index
Journal :
Iranian Journal of Parasitology
Publication Type :
Academic Journal
Accession number :
181243309