鸡源高黏附性乳酸菌的筛选及其发酵条件优化.

The purpose of the study was to screen and obtain chicken-derived lactic acid bacteria with high adhesion, excellent probiotic properties and high activity so as to lay a foundation for the development of chicken-derived probiotic preparations. The host-derived lactic acid bacteria were isolated from the intestinal tract of healthy free-range chickens and the surface adhesion characteristics of the isolated strains such as surface hydrophobicity, bacterial self-agglutination and co polymerization as well as the adhesion effect on intestinal mucus in vitro were studied. The response surface method was used to optimize the significant influencing factors in the fermentation conditions of high adhesion lactic acid bacteria and the optimal fermentation conditions were obtained. The results showed that a strain of Lactobacil⁃ lus reuteri Lac-R6 was isolated from chicken intestine and the adhesion efficiency in vitro was 29.47%. The survival rate in the simulated gastrointestinal fluid was 80.11% and it had a significant antibacterial effect on pathogenic E. coli 1.505 (P<0.05). The adhesion efficiency was significantly higher than that of other lactic acid bacteria isolates and it had good gastrointestinal tolerance and probiotic characteristics. The fermentation conditions of Lactobacillus reuteri L6 were optimized. The optimal fermentation conditions were as follows: initial pH 6.73, yeast extract powder addition 6.63 g/L, and glucose addition 28.85 g/L. The number of effective living bacteria could reach 8.56 × 109 CFU/mL by optimization. Lactobacillus reuteri L6 that isolated from chicken intestine is a high-adhesion host-derived lactic acid bacteria with good colonization advantages and probiotic properties which helps it to play a probiotic role in the host. [ABSTRACT FROM AUTHOR]